Smart NGS Solutions and Bioinformatics for Research and Health
From minute amounts of material to high resolution data: full service or kits for all your samples
Massive Analysis of cDNA Ends = MACE = 3′ mRNA-Seq:
High Resolution Gene Expression at only 10% of RNA-Seq sequencing costs – Service or Kit
MACE is an improved variant of “3′ single end mRNA Seq” or “Tag-Seq” that is based on sequencing only one single molecule or “tag” per transcript. Therefore, each NGS read represents one single transcript. In contrast to most other Tag-Seq methods, in MACE-Seq, each molecule is barcoded with a unique sequence (“TrueQuant” -unique molecular identifiers) for PCR bias elimination (patented). MACE-Seq reveals gene expression, allele frequencies and alternative polyadenylation at only a fraction of the sequencing costs of regular RNA-Seq. Our service optionally includes all steps, from RNA-extraction to bioinformatics. MACE-Seq is also available as a kit for your own lab.
Because MACE data has some special characteristics, GenXPro includes the bioinformatics analysis both for the service and the kit. Therefore, you do not need to set up your own bioinformatics and can profit from our constantly improved and widely used bioinformatics pipeline.
Besides the raw-data, you receive ready-to-use results tables containing NCBI or ENSEMBL IDs and descriptions, expression values, p-values for differential expression, Fold Changes, SNPs, etc., which can conveniently be analyzed e.g. with other bioinformatics analysis tools or Excel.
In addition, GenXPro provide Gene Ontology (GO) Enrichment Analyses and plots and graphs which can be easily accessed and browsed using our Web-Interface (http://tools.genxpro.net).
Data is provided via WEB-access or can be shipped on a hard-disk.
The MACE-Seq Kit
GenXPro’s highly successful MACE-Seq service is now also available as a kit. It contains all ingredients to perform MACE-Seq. In contrast to other “3’mRNA Seq” or “Tag-Seq” approaches the MACE-Seq Kit includes patented TrueQuant method (molecular barcodes) to avoid PCR-bias, for the generation of qualitative and quantitative accurate data on any Illumina sequencing machine. MACE-Seq allows for accurate and cost efficient high-resolution gene expression profiling, genotyping in transcripts, allele frequency determination and the analysis of alternative polyadenylation. Up to 96 samples can be processed in parallel.
The MACE kit comes with bioinformatics-vouchers for a convenient analysis of the data on GenXPro’s servers. As MACE data have some specificities, GenXPro developed an optimized analysis pipeline that you can access using the vouchers. Simply upload your data using the provided voucher-codes to servers. GenXPro provide complete result tables compatible with Excel, as well as plots of your data and Gene Ontology enrichment analyses. The raw and the refined data is accessible via our easily understandable web interface at http://tools.genxpro.net.
Further bioinformatics analyses are possible e.g. SNP-analysis, alternative polyadenylation or allele frequency determination (see Bioinformatics)
Therefore you do not need bioinformatians nor special hardware for the detailed analysis of the complex data.
Analyse 6 samples simultaneously on the MiSeq or 96 samples on a HiSeq or NextSeq500
The kit is available as 6x MACE version for up to 6 MACE libraries on the Illumina MiSeq platform and a 24x version for the Illumina HiSeq or NextSeq.
With further indices, several kits can also be combined for example to reach 4×24 samples.
Required input material: >10 ng totalRNA (can vary depending on tissue and quality of RNA)
Number of samples 1 – 96 (depending on Kit)
Time for sample preparation of 96 samples: 2 days
Kit for MiSeq (Illumina), Single End Sequencing
6x Kit: 390.00 EUR (cat. no. 16046.1)
Bioinformatics voucher 6x: 360.00 EUR (cat. no.16046.2)
Kit for HiSeq or NextSeq (Illumina), Single End Sequencing
24x Kit: 1,320.00 EUR (cat. no.160424.1)
2x Additional Post-Pool reactions1: 80.00 EUR (cat.no. 16040.1)
4x Additional Post-Pool reactions1: 140.00 EUR (cat.no. 16040.2)
Bioinformatics voucher 24x*: 1,080.00 EUR (cat. no.160424.2)
Additional adapter set to combine up to four kits: 160.00 EUR (cat. no.160424.3)
(All prices without tax)
*the bioinformatics is limited to model organisms and includes annotation, quantification, differential gene expression (DE) analyses of pairwise comparisons (p-values for DE, log 2 Fold Changes) and GO enrichment analyses in our MACE cloud server. Data is accessible as downloadable .xls table and through our web interface at http://tools.genxpro.net
1Pooling strategy: One advantage of the MACE kit is that the samples are pooled after generating barcoded cDNAs. The 6x and 24x kits are designed to pool up to 6 and 24 libraries, respectively. The kit contains reactions to generate up to 6 or 24 libraries in the pre-pooling step and one post-pool reaction in the 6x kit and two post pool reactions in the 24x kit. For additional pools (e.g. for only two samples), additional post-pooling reagents can be purchased separately (cat.no. 16040.1 and 16040.2).